Sperm quality in male <Emphasis Type="Italic">Barbus barbus</Emphasis> L. fed different diets during the spawning season

Sperm quality of Barbus barbus L. was compared among the three following dietary regimes: Group A, fed 100% commercial diet (Karpico™ containing 33% crude protein and 6% fat), Group B, fed 78% commercial diet and 22% frozen chironomid (Chironomus plumosus) larvae, and Group C, fed 56% commercial diet and 44% frozen chironomid larvae. Concentrations of polyunsaturated fatty acids (PUFAs) in Group A, B, and C were 39.1, 42.0, and 44.6, respectively, as a percentage of total fatty acids. Sperm morphology, volume, concentration and motility, total number of spermatozoa, and osmolality of the seminal plasma were compared during the spawning season. Dietary regime did not influence sperm volume, concentration, or total number of spermatozoa, osmolality of seminal plasma, or the percentage of motile sperm, but significantly affected sperm morphology (except for anterior and posterior parts of the midpiece) and sperm velocity (P < 0.05). Groups B and C showed similar sperm characteristics during the spawning season compared to Group A. Almost all parameters changed either among or within groups during the spawning season, suggesting differences in terms of the optimal time for sperm collection. The best time for sperm collection was March for Group A, but April for Groups B and C, when the osmolality of the seminal plasma measured 289 mOsmol kg⁻¹ and sperm motility was maximal. Spermatogenesis, hydration, and cell decomposition were confirmed as the three major parameters controlling sperm characteristics during the spawning season. The possible correlation between sperm morphology and motility requires further study.     

Within-field refugiums of Russian wheat aphid, Diuraphis noxia (Kurdj.) in cereals (Hom., Aphididae)

An analysis of the Russian wheat aphid (RWA) (Diuraphis noxia Kurdj.), overpopulated due to extraordinary weather conditions (2000) in the Czech Republic, enabled the identification of several within-field situations ouside of the routine approach. This group, classified as within-field refugium, includes variation due to field relief, some injuries caused by the use of machinery, the unharvested field margins and, last but not least, the volunteer plants. Differences between the RWA populations in these situations and the normal grove are presented and recommended to be taken into consideration in the research on RWA population dynamics and movement.     

A temperature-driven model to simulate the population development of the Plum Fruit Moth,Cydia funebrana (Treit.)

A simulation model of the flight activity ofCydia funebrana was constructed on the basis of data on male captures in pheromone traps placed in a plum orchard in Central Bohemia in 1988 to 1990. The model was verified from 1991 to 1993 as a method of predicting the course of the emergence flight activity of particular generations in dependence on degree day values. The nonlinear model based on Richards' transformation was more suitable for the simulation than the linear one based on probit transformation. A technique has been proposed to use the model ofC. funebrana flight activity for optimal timing of ovicide applications based on fenoxycarb and diflubenzuron in systems of integrated pest management in plum orchards.With 3 figures and 4 tables     

Flight patterns of the peach twig borer,Anarsia lineatella zell. (Lep., Gelechiidae) in central europe as observed using pheromone traps

Pheromone traps were used for monitoring phenology of the peach twig borer,Anarsia lineatella, in Central Bohemia. The pest has two generations a year, the first one (owerwintering) is usually more numerous than the second one. The peak of the flight of the first generation begins by day-degrees (DD) 400–450 °C and the peak of the flight of the second generation at DD 900–960 °C. DD data can be used for supervised control both in the case of timing the chemical treatment and for deciding on using pheromones for male confusion. The suitable term for chemical treatment in the given locality in Central Bohemia is DD 360°C (above the developmental threshold 10 °C). The injury to fruits and shoots of peach trees similar to that caused byA. lineatella does also the oriental fruit moth,Cydia molesta. According to very low catches in pheromone trapsC. molesta does not seem to be economically important in Central Bohemia at present.With 2 tables and 3 figures     

Relation between combustion heat and chemical wood composition during white and brown rot

Summary The results of respective investigations indicate that during white rot the combustion heat of wood (Q_exp (J/g)) remained virtually unchanged and at the greatest extent of decomposition it was in the range also found in non-rotten wood. During brown rot the combustion heat of wood samples increased, beginning at a weight loss of 20–30%. The difference between Q_exp and the weighted mean of combustion heat values of isolated chemical wood components (Q_cal) were neglectable. If Q_exp values measured for each sample were taken as 100%, then Q_cal averaged from 101.1–105.6% and from 97.4–108.2% during white and brown rot, respectively. The Q_cal calculation error is expressed as coefficient k; it varies depending on the accuracy and selectivity of the separation methods used.     

Generationszahl der RübenfliegePegomyia hyoscyami betae Curt.

Summary During the years 1959–1961 the number of generations was followed in Spinach Leaf Miner (Pegomyia hyoscyami betae Curt.) in central Bohemia at 310 m above sea level and in the year 1961 in southern Bohemia at 450 m. The larvae and the eggs were studied on the leaves at regular 10–14 days' intervals by controlling each plant and images by means of a sweeping method. There have been acertained two generations in the year 1959 and three generations in the year 1961 in central Bohemia and two generations in the year 1961 in southern Bohemia. The occurence of every generation of larvae on the suggar-beet was separate and the previous and the consecutive generation did not overlop.The origin of the erroneous date on 6–7 generations of this species (Rambousek, Neuwirth, 1931) has been also cleared up in this article. This statement has been taken over by Sorauer (1953) from the Review of Applied Entomology (1931) and has been lately mentioned by many authors. F. Neuwirth gave a personal information, that the statement on 6–7 generations occurence of Spinach Leaf Miner has been found on the basis of theoretical calculation (from the lenght of one generation in spring and not on the basis of the facts found in natural conditions).

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Résumé En 1959–1961 le nombre des générations de la mouche de la betterave a été observé dans la Bohème Centrale á l'altitude de 310 m audessus de la mer et en 1961 dans le Sud de la Bohème á l'altitude de 450 m. Les larves et les oeufs ont été observé sur les feuilles dans les intervalles de 10–14 jours regulierement effectués sur chaque plante et imagines en particulier par le filet fauchior. En 1959 deux générations et en 1960 et 1961 trios géné rations dans la Bohéme Centrale, ainsi que en 1961 deux générations dans le Sud de la Boheme ont été constates. La prence de chaque génération des larves sur la betterave aété separée et les générations consecutives ne malaient pas presque jamais avec les précedents.Dans cet travail l'auteur a clarifié comment une donnée fautive sur 6–7 générations de cette espéce est survenu (Rambousek et Neuwirth, 1931). Cette donnée orginante de Review of Applied Entomology [1931] a été reprise par Sorauer (1953) et elle a été ensuite récemment mentionée par d'autres auteurs. Selon une déclaration personelle de Mr. F. Neuwirth les donées des 6–7 générations de la mouche de la betterave en Tschécoslovaquie ent été acquis par une évaluation théoretique (se basant sur la durée de la génération du printemps et non sur les resultats des observations réelles dans les conditions naturelles.

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1959–61 . (310 . ) 1961 . (450 . ). 10–14 , . 1959 . 2 , 1960 . — 3, 1961 . — 2 . , . , 6–7 (Rambousek, Neuwirth 1931). . . 6–7 , , .

     

Bejlomorky (Gallmücken.)

Ohne Zusammenfassung     

Supercritical fluid extraction of bioavailable amino acids from soils and their liquid chromatographic determination with fluorometric detection

A new procedure with supercritical CO2 modified with 0.5 mL of water and 0.75 mL of 0.1 M HCl in situ and 0.75 mL of water on-line at 15 MPa and 50 degrees C for 45 min was applied for the extraction of bioavailable amino acids from soil samples. Total extraction time was 60 min, but more favorable conditions are even possible for selected groups of amino acids. All analytes were trapped into 20 mL of methanol with satisfactory recovery (94-104%) and determined using high-performance liquid chromatography with fluorometric detection on a Zorbax Eclipse column (4.6 x 75 mm, 3.5 microm) with Na2HPO4 and acetonitrile/methanol/water as a mobile phase. Linear calibration curves were obtained (r > 0.999 except 0.99823 for Ile) with lower limits of detection (S/N = 3) in the range from 1.54 pg (Gly) to 13.5 pg (Cy2) or from 18.6 fmol (Ser) to 64.8 fmol (Lys). Validation and repeatability data are also given. Comparable results were obtained with a robust, commonly used extraction method (0.5 M ammonium acetate, 60 min in shaker, followed by filtration and lyophilization). Limiting values of artificial release of amino acids were also determined for each soil sample to eliminate any false results to ensure that all extracted amino acids originate from soil solution and exchangeable bound positions of soil samples.